Mechanism of Action of Nalidixic Acid on ... | 合法藥品大搜索

由NBouck著作·1970·被引用42次—Whennalidixicacid,aspecificandeffectiveinhibitorofcellulardeoxyribonucleicacidsynthesis,isaddedtoconjugatingbacteriaatanytimeduringmating,itstops ...

J Bacteriol. 1970 Jun; 102(3): 688–701.

aDepartment of Microbiology, Yale University, New Haven, Connecticut 06510

1 This report is part of a dissertation presented by the senior author in partial fulfillment of the requirements for the Ph.D. degree from Yale University.

When nalidixic acid, a specific and effective inhibitor of cellular deoxyribonucleic acid synthesis, is added to conjugating bacteria at any time during mating, it stops genetic transfer provided the donor bacterium is sensitive to the drug. When this inhibition is released by the removal of the nalidixic acid, transfer does not resume at the point on the chromosome where it was stopped, but begins again at the transfer origin. Curves relating the effects of various low doses of nalidixic acid to the frequency of recombination reveal that several “hits” are necessary to inhibit recombinat...