Optimizing Purification of Histidine | 合法藥品大搜索

組胺酸（英語：Histidine， C6H9N3O2）簡寫為His或H，α胺基酸結合咪唑官能團。是存在於蛋白質之中最普遍的23種胺基酸之一。初以為只針對嬰幼兒是必需 ... Read More

Properties of the imidazole side chain — The conjugate acid (protonated form) of the imidazole side chain in histidine has a pKa of approximately 6.0. Read More

結合在介質上的His-tag 蛋白可以通過提高緩衝液中的咪唑(Imidazole) 濃度進行競爭性洗脫，從而得到較高純度的His-tag 蛋白。在親和純化實驗中的使用最為廣泛的 ... Read More

As the number of histidine residues increases, the elution buffer requires a higher concentration of imidazole to remove bound proteins from the column. Read More

Optimizing using imidazole — The examples below show how changes in imidazole concentration during binding affect the purity of the histidine-tagged ... Read More

由 RNVK Deepak 著作 · 2016 · 被引用 18 次 — Although histidine's imidazole nitrogens (Nδ and Nε) are known to participate in hydrogen bond (HB) interactions as an acceptor or a donor, a ... Read More

The imidazole group functions as a nucleophile, or a general base, in the active sites of many enzymes, and may bind metal ions. Histidine is nonessential in ... Read More

Histidine, an essential amino acid, has as a positively charged imidazole functional group. The imidazole makes it a common participant in enzyme catalyzed ... Read More

由 AT Kessler 著作 · 2020 · 被引用 8 次 — Histidine's imidazole side chain is unique amongst amino acids, giving rise to its aromaticity and amphoteric properties at physiologic pH. Read More

由 JA Bornhorst 著作 · 2000 · 被引用 669 次 — Histidine is the amino acid that exhibits the strongest interaction with immobilized metal ion matrices, as electron donor groups on the histidine imidazole ring ... Read More