[16] Purification of Proteins Using Polyhistidine Affinity Tags | 合法藥品大搜索

由JABornhorst著作·2000·被引用669次—Histidineistheaminoacidthatexhibitsthestrongestinteractionwithimmobilizedmetalionmatrices,aselectrondonorgroupsonthehistidineimidazolering ...

Methods Enzymol. Author manuscript; available in PMC 2010 Jul 25.

Published in final edited form as:

PMCID: PMC2909483

NIHMSID: NIHMS214279

The expression and subsequent purification of recombinant proteins are widely employed in biochemical studies. A powerful purification method involves the use of peptide affinity tags, which are fused to the protein of interest and used to expedite protein purification via affinity chromatography.1,2 A widely employed method utilizes immobilized metal-affinity chromatography (IMAC) to purify recombinant proteins containing a short affinity tag consisting of polyhistidine residues. IMAC is based on the interactions between a transition metal ion (Co2+, Ni2+, Cu2+, Zn2+) immobilized on a matrix and specific amino acid side chains. Histidine is the amino acid that exhibits the strongest interaction with immobilize...